特別講演会御案内

 

演題: EFFECTS OF POLYMER CHEMISTRY ON DNA SEQUENCING SEPARATIONS BY MICROCHANNEL ELECTROPHORESIS

 

演者: Prof. Annelise E. Barron

(Northwestern University)

 

日時: 2000年 11月28日(火) 午後 4:00〜5:00

 

場所: 徳島大学薬学部第1会議室

The chemical and physical properties of entangled polymer solutions are critical for their performance as sequencing matrices for microchannel electrophoresis, affecting both DNA resolving capability and matrix viscosity for pressurized capillary loading. We have systematically compared matrices based on polymers and copolymers of acrylamide, di-methylacrylamide (DMA) and diethylacrylamide (DEA). Viscosities of 7% polymer solutions decrease by orders of magnitude with increasing polymer hydrophobicity, while hydrophilic polymers exhibit more pronounced reductions in viscosity with increased shear. As sequencing matrices under identical CE conditions, the longest read length was produced with linear polyacrylamide (LPA) while linear polyDMA (PDMA) gave ~ 100 fewer readable bases. Read lengths with DMA/DEA copolymers were lower, and decreased with increasing DEA content. This study highlights the importance of polymer hydrophilicity for DNA sequencing matrices, through formation of robust, entangled polymer networks.

 

マイクロチップやキャピラリー電気泳動によるDNAシークエンシングおよびSNP解析用のポリマー開発の第一人者であるBarron教授に講演いただきます。特に、熱感応性ポリマー等新規ポリマー開発の現状と将来について分かりやすく御解説いただく予定です。多くの、先生方、院生・学生のみなさんの御参加をお待ちしております。

 

連絡先: 徳島大学薬学部薬品物理化学教室

      馬場嘉信

    Tel: 0886-33-7285

    Fax: 0886-33-9507

    E-mail: ymttbaba@ph.tokushima-u.ac.jp